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目的应用生物信息学数据库和工具,对现有的H3N2亚型人甲型流感病毒全球分离株的H3A1抗原序列进化规律进行分析研究。方法下载NCBI Genbank和流感病毒数据库中全部的甲型流感病毒H3A1序列,首先用两步聚类法进行样本拆分,随后分类绘制出完整的进化树。结果人H3A1序列呈现出单一主干的进化趋势,随着时间的推移,进化树结构和进化模型相关参数均呈现出一定的变化规律,关键变异株的出现则无明显的地域分布特征。结论人流感病毒H3A1抗原的进化主要是病毒抗原漂移和人类免疫选择相互作用的结果,新变异株的出现并未出现明显的地域倾向性,中国华南地区不应当被认为是H3亚型新变异株的发源地。
Objective To analyze the evolution of the H3A1 antigen sequence of the existing H3N2 subtype human influenza A virus isolates using bioinformatics databases and tools. Methods All H3A1 sequences of influenza A virus in NCBI Genbank and the influenza virus database were downloaded. The two-step clustering method was used to split the samples and then the complete phylogenetic tree was classified. Results The human H3A1 sequence showed a single trunk evolution trend. With the passage of time, the evolutionary tree structure and evolutionary model related parameters showed some changes, while the emergence of the key mutant strains showed no obvious geographical distribution. Conclusions The evolution of H3A1 antigen of human influenza virus is mainly the result of the interaction between viral antigen drift and human immune selection. The emergence of new mutants does not show any obvious geographical predominance, and southern China should not be considered as a new mutant of H3 subtype The birthplace of