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目的:建立HPLC测定冠心七味片中丹参素含量的方法。方法:采用Shim-pack CLC-ODS(4．6 mm×150 mm)色谱柱;流动相为甲醇-乙腈-磷酸三乙胺水溶液(5:2:93),检测波长为281 nm,流速为1．0 ml·min-1。结果:丹参素在0．08-0．73μg范围内呈良好的线性关系(r=0．999 7),平均回收率为97．22％,RSD为1．66％。结论:方法较简单、快速、分离度好,结果稳定,可作为本品的质量控制方法。
Objective: To establish a HPLC method for the determination of Danshensu in Guanxin Qiwei Tablets. Methods: A Shim-pack CLC-ODS (4.6 mm×150 mm) column was used. The mobile phase was methanol-acetonitrile-triethylamine aqueous solution (5:2:93). The detection wavelength was 281 nm and the flow rate was 1 .0 ml·min-1. Results: Danshensu showed a good linearity in the range of 0.08-0.73 μg (r=0.999 7). The average recovery was 97.22% and the RSD was 1.66%. Conclusion: The method is simple, rapid, and has good resolution and stable results. It can be used as a quality control method for this product.