目的：测定陕西地区丙型肝炎病毒的Ｃ区基因序列，为进一步研究ＨＣＶ的流行病学和开展ＨＣＶ的诊治打下基础．方法：自行设计合成了两条正向引物和两条反向引物，用反转录ＰＣＲ（ＲＴＰＣＲ）法从陕西地区１例ＮＡＮＢ型肝炎患者血清中扩增出４３６ｂｐ的片段，将此片段克隆于ＰＧＥＭ７Ｚｆ（＋）中用全自动序列分析仪测序．结果：测得４３６ｂｐ的核苷酸序列，将所得核苷酸序列及由它们所编码的相应氨基酸序列与ＨＣＶＪ６，ＨＣＶＪ８，ＨＣＶＨｅｂｅｉ，ＨＣＶＨｕｎａｎ，ＨＣＶ１比较，显示所分离ＨＣＶ在核苷酸水平上与以上分离株的同源性分别为９５７％，８７２％，８３９％，８３９％和８２７％；在氨基酸水平上的同源性分别为９７７％，９４７％，９０８％，９０１％和９０１％．结论：所分离株与ＨＣＶＪ６同属２ａ型 Objective: To determine the C region gene sequence of hepatitis C virus in Shaanxi province and lay the foundation for further study of HCV epidemiology and diagnosis and treatment of HCV. Methods: Two forward primers and two reverse primers were designed and synthesized. A 436 bp fragment was amplified from the serum of a patient with NANB hepatitis in Shaanxi Province by reverse transcription PCR (RTPCR) Clone in PGEM  7Zf (+) with automatic sequence analysis of sequencing. Results: The nucleotide sequence of 436bp was measured, and the nucleotide sequences obtained and the corresponding amino acid sequences encoded by them were compared with HCV-J6, HCV-J8, HCV-Hebei, HCV-Hunan and HCV- Isolation of HCV at the nucleotide level with the above isolates were 95.7%, 872%, 839%, 839% and 827%, respectively. At the amino acid level, homology The results were 977%, 947%, 908%, 901% and 901% respectively. Conclusion: The isolated strains and HCV J6 belong to 2a type 