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[目的]防止漏检赖氨酸脱羧酶阴性的伤寒菌株。[方法]2012年5月在一发热病人血液标本中检出1株沙门菌,用沙门菌显色培养基、强、弱选择培养基分离,对菌株进行系统生化、API20E、美国BD全自动细菌鉴定仪及血清学试验确认。[结果]分离培养、生化特性、血清学试验均符合伤寒沙门菌,只有赖氨酸脱羧酶阴性。经微量生化等系统鉴定为1株赖氨脱羧酶阴性的伤寒菌。[结论]必须注意鉴别赖氨酸脱羧酶阴性的伤寒菌。
[Objective] To prevent undetected lysine decarboxylase-negative typhoid strains. [Method] A strain of Salmonella was detected in blood samples of a febrile patient in May 2012. Salmonella was detected by Salmonella chromogenic medium and strong and weak selective medium. The biochemical, API20E, Identification and serological test confirmed. [Result] Isolation and culture, biochemical characteristics and serological tests all accorded with Salmonella typhi. Only lysine decarboxylase was negative. The micro-biochemical system identified as a lysine decarboxylase negative typhoid bacteria. [Conclusion] It is necessary to pay attention to identify lysine decarboxylase-negative typhoid bacteria.