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目的:观察不同浓度17β-雌二醇(E2β)处理时,人胃癌BGC823细胞生长情况以及雌激素受体ERα36表达的变化,探讨E2β在胃癌生长调节中的作用及相关机制。方法:BGC823细胞经10-10,10-11和10-12 mol/L浓度的E2β处理24h和48h。细胞生长通过WST1方法检测。RT-PCR,Western blot及灰度分析法检测ERα36mRNA水平及蛋白水平变化,其平均光密度值通过t检验分析,应用免疫荧光染色方法检测ERα36蛋白在细胞中的位置变化。结果:E2β可促进胃癌BGC823细胞生长,但随着其浓度的上升,E2β的促生长能力下降。E2β可促进ERα36mRNA表达,该促进作用具有浓度依赖性。E2β处理BGC823细胞24h时,ERα36蛋白表达上升,48h时,ERα36蛋白表达下降。ERα36蛋白定位于细胞膜,E2β对BGC823细胞ERα36蛋白定位无明显影响。结论:E2β可促进胃癌细胞BGC823生长。E2β-ERα36可能通过膜信号通路参与了胃癌细胞的生长调节。
OBJECTIVE: To observe the growth of human gastric cancer cell line BGC823 and the expression of estrogen receptor ERα36 in different concentrations of 17β-estradiol (E2β), and to explore the role of E2β in the regulation of gastric cancer growth and its related mechanisms. Methods: BGC823 cells were treated with E2β at concentrations of 10-10, 10-11 and 10-12 mol / L for 24 h and 48 h respectively. Cell growth was measured by the WST1 method. The changes of ERα36 mRNA and protein levels were detected by RT-PCR, Western blot and gray-scale analysis. The mean optical density was analyzed by t test. The changes of ERα36 protein in the cells were detected by immunofluorescence staining. Results: E2β could promote the growth of gastric cancer BGC823 cells, but with the increase of its concentration, the growth promoting ability of E2β decreased. E2β can promote ERα36 mRNA expression in a concentration-dependent manner. E2β treated BGC823 cells 24h, ERα36 protein expression increased 48h, ERα36 protein expression decreased. ERα36 protein located in the cell membrane, E2β BGC823 cells ERα36 protein localization had no significant effect. Conclusion: E2β can promote the growth of BGC823 gastric cancer cells. E2β-ERα36 may participate in the growth regulation of gastric cancer cells through membrane signaling pathway.