目的探讨维甲酸（ＲＡ）对ＨＬ６０细胞的增殖抑制和诱导分化作用与细胞周期素依赖性激酶（ＣＤＫ）活性变化的相关性。方法用５×１０－６ｍｏｌ／Ｌ全反式维甲酸（ＡＴＲＡ）或芳维甲（ＡＥ）处理ＨＬ６０细胞１～４天，在观察细胞生长、四氮唑蓝（ＮＢＴ）还原实验以及细胞周期分析的基础上，用组蛋白Ｈ１激酶活性分析技术检测ＣＤＫ２的活性，免疫沉淀法分析结合于ＣＤＫ２和ＣＤＫ４上的相应的细胞周期素（ｃｙｃｌｉｎ）Ｅ和Ｄ的量。结果ＨＬ６０细胞在ＡＴＲＡ或ＡＥ作用下，细胞增殖减慢，对ＮＢＴ的还原能力明显增强，９０％左右的细胞被阻滞在Ｇ１／Ｇ０期；在Ｇ１到Ｓ期转换过程中起关键作用的ｃｙｃｌｉｎＥ／ＣＤＫ２的活性显著下降，结合于ＣＤＫ４上的ｃｙｃｌｉｎＤ１的量减少。结论ＲＡ抑制ＨＬ６０细胞增殖并诱导其分化可能与ＣＤＫ２和ＣＤＫ４的活性下降有密切关系 Objective To investigate the relationship between the inhibitory effect of retinoic acid (RA) on the proliferation and differentiation of HL60 cells and the changes of the activity of cyclin dependent kinase (CDK). Methods HL60 cells were treated with 5 × 10-6 mol / L all-trans retinoic acid (ATRA) or Adenoviruses (AE) for 1 ~ 4 days. The cell growth, NBT reduction, Based on the cycle analysis, the activity of CDK2 was assayed using the histone H1 kinase activity assay and the amount of the corresponding cyclin E and D bound to CDK2 and CDK4 was analyzed by immunoprecipitation. Results HL-60 cells under the action of ATRA or AE, cell proliferation slowed down, the reduction of NBT was significantly enhanced, about 90% of cells were arrested in the G1 / G0 phase; in the G1 to S phase transition process plays a key role The cyclinE / CDK2 activity was significantly decreased, and the amount of cyclinD1 bound to CDK4 decreased. Conclusion RA inhibition of proliferation and induction of HL 60 cell differentiation may be related to the decline in the activity of CDK2 and CDK4