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实验采用了愈伤组织和增强腋芽生枝能力两种途径对长寿花进行组织培养。愈伤组织途径:基本培养基为MS,最佳外植体为叶片,诱导愈伤培养基中PGR最佳浓度配比为:6-BA(2.0mg/L)+NAA(0.4mg/L)+2,4-D(0.8mg/L),胚状体的分化培养基中不加入生长调节物质效果最好。增强腋芽生枝能力途径:基本培养基为MS,外植体为带节的茎段,诱导丛生芽培养基中PGR最佳浓度配比为:6-BA(3.0mg/L)+NAA(0.4mg/L)。生根培养中,最佳培养基为1/2MS+NAA(0.2mg/L)。生根后,待根长到3~5cm即可炼苗、移栽。
The experiment used callus and enhanced axillary bud twig two ways to tissue culture of kalanchu flower. The callus pathways: the basic medium was MS, and the best explant was leaf. The optimum concentration of PGR in callus induction medium was 6-BA (2.0mg / L) and NAA (0.4mg / L) + 2,4-D (0.8mg / L). The differentiation medium of embryoid bodies did not have any growth regulator. Pathways of enhancing axillary bud growth: The basic medium was MS, and the explants were stem segments. The optimal concentration of PGR in the induced bud clusters was 6-BA (3.0 mg / L) and NAA / L). In rooting culture, the best culture medium was 1 / 2MS + NAA (0.2mg / L). After rooting, until the root length of 3 ~ 5cm can Lianmiao, transplanting.