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目的了解温州市食源性金黄色葡萄球菌的耐药趋势、肠毒素及其基因分布情况,为金黄色葡萄球菌食源性疾病的预防控制提供参考。方法采用纸片法进行药敏试验,用mini-VIDAS法和PCR法分别进行肠毒素及其基因的检测。结果 100株金黄色葡萄球菌,其中97株(97.00%)均有不同程度的耐药,对青霉素耐药率最高(92.00%),其次为甲氧苄啶(42.00%)、红霉素(34.00%),多重耐药率达29.90%;43株(43.00%)肠毒素阳性,奶粉及餐食阳性率较高;40株检出肠毒素基因,总检出率为40.00%,其中seb基因检出率最高。形成16种基因组合,以seb基因型为主(10.00%),同时检出2种及以上肠毒素基因的菌株占20.00%。结论温州市食源性金黄色葡萄球菌具有较强耐药性,且其产肠毒素率及毒素基因携带率较高,存在潜在的食品安全隐患。
Objective To understand the drug resistance trend, enterotoxin and its gene distribution of foodborne Staphylococcus aureus in Wenzhou City and provide reference for the prevention and control of foodborne disease of Staphylococcus aureus. Methods The antibacterial susceptibility test was carried out by the paper method, and the enterotoxin and its gene were detected by mini-VIDAS and PCR respectively. Results 100 strains of Staphylococcus aureus, 97 strains (97.00%) had different degrees of resistance, the highest rate of resistance to penicillin (92.00%), followed by trimethoprim (42.00%), erythromycin %), The rate of multidrug resistance was 29.90%; 43 strains (43.00%) were positive for enterotoxigenin, the positive rate of milk powder and meal were higher; the detection rate of enterotoxin gene was 40.00% in 40 strains, of which seb The highest rate. A total of 16 genotypes were found, which accounted for seb (10.00%), and 20.00% were also detected when two or more enterotoxin genes were detected. Conclusion The food-borne Staphylococcus aureus in Wenzhou is highly resistant, and its production of enterotoxins and high rate of toxin gene carrying has potential food safety problems.