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研究雌二醇和它莫西芬对培养的小鼠腹腔巨噬细胞的影响，发现雌二醇能够诱导巨噬细胞产生典型的细胞程序性死亡的形态学改变和特征性的ＤＮＡ“梯形”结构，而它莫西芬能够抑制雌二醇诱导细胞程序性死亡的作用。用１μｍｏｌ／Ｌ，１００ｎｍｏｌ／Ｌ，１０ｎｍｏｌ／Ｌ的１７，β－雌二醇处理巨噬细胞１２ｈ后出现典型的ＤＮＡ“梯形”结构，呈剂量依赖方式。１０μｍｏｌ／Ｌ的它莫西芬能够有效抑制１μｍｏｌ／Ｌ的１７，β－雌二醇诱导的巨噬细胞程序性死亡。Ｓｔａｕｒｏｓｐｏｒｉｎｅ和放线菌酮能够抑制雌二醇诱导巨噬细胞产生的典型的ＤＮＡ“梯形”结构的形成。
To study the effects of estradiol and tamoxifen on cultured murine peritoneal macrophages, estradiol was found to induce macrophages to produce morphological changes typical of programmed cell death and characteristic DNA “trapezoidal” structures, And tamoxifen can inhibit the role of estradiol-induced apoptosis. The macrophages treated with 1μmol / L, 100nmol / L and 10nmol / L 17, β-estradiol showed typical DNA “trapezoid” structure in a dose-dependent manner. Tamoxifen at 10 μmol / L effectively inhibited 1 μmol / L 17, β-estradiol-induced apoptosis of macrophages. Staurosporine and cycloheximide inhibit the formation of a typical “ladder” structure of estradiol-induced macrophages.