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目的 :考察大孔吸附树脂吸附纯化效果的影响因素 ,为在中药纯化研究中应用吸附纯化法、优化吸附纯化条件提供参考。方法 :以黄连水提液为样本 ,小檗碱为代表成分 ,研究树脂和中药提取液两方面对吸附纯化效果的影响。结果 :树脂的种类、提取液的浓度、提取液中盐溶液的种类和用量及外环境的温度都会不同程度地影响吸附纯化的过程和结果。结论 :用吸附纯化法纯化中药制剂 ,应针对具体样品进行影响因素综合考察 ,方能筛选出较佳的纯化条件。
Objective: To investigate the factors affecting the adsorption and purification of macroporous resin, and to provide references for the application of adsorption and purification methods and optimization of adsorption and purification conditions in the purification of traditional Chinese medicine. Methods: Taking Coptis chinensis water extract as sample and berberine as representative component, the effect of resin and Chinese herbal extract on adsorption purification was studied. Results: The type of resin, the concentration of the extract, the type and amount of the salt solution in the extract, and the temperature of the external environment all affected the adsorption purification process and the results in different degrees. Conclusion :The purification of traditional Chinese medicine preparations by adsorption and purification method should be based on a comprehensive survey of the influencing factors of specific samples in order to select the best purification conditions.