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研究了稀土元素对细菌抗性及细胞壁结构的影响。用抑菌圈法测定了硝酸镧和硝酸铈对枯草芽孢杆菌和大肠杆菌抗性的影响,并用傅里叶变换红外光谱研究了细菌细胞壁的结构。适当浓度(150 mg/L~200 mg/L)硝酸镧和硝酸铈可以缩小这两种细菌对0.135%升汞、9%苯酚、75%乙醇和80万单位/毫升青霉素溶液的抑菌圈,抑菌圈变小表明细菌抗性增强,红外光谱显示硝酸镧和硝酸铈影响了酰胺的C=O伸缩振动(称酰胺Ⅰ峰),表明细菌细胞壁肽聚糖结构受到了影响。细菌抗性的改变可能与细胞壁肽聚糖结构受到影响有关。
The effects of rare earth elements on bacterial resistance and cell wall structure were studied. The effect of lanthanum nitrate and cerium nitrate on the resistance of Bacillus subtilis and Escherichia coli was determined by inhibition zone method. The structure of bacterial cell wall was studied by Fourier transform infrared spectroscopy. The inhibition zone of 0.135% mercuric chloride, 9% phenol, 75% ethanol and 800,000 units / ml penicillin solution of these two kinds of bacteria can be reduced by suitable concentration of lanthanum nitrate and cerium nitrate from 150 mg / L to 200 mg / Bacteriostatic zone smaller indicates increased bacterial resistance, infrared spectroscopy showed that lanthanum nitrate and cerium nitrate affect the amide C = O stretching vibration (known as amide Ⅰ peak), indicating that bacterial cell wall peptidoglycan structure has been affected. Changes in bacterial resistance may be related to the influence of cell wall peptidoglycan structure.