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目的:探讨脑缺血再灌注损伤(CIRI)过程中小胶质细胞(MG)向树突状细胞(DC)转化情况;方法:线栓法建立CIRI小鼠模型;免疫荧光方法检测MG转化为DC情况;流式细胞仪检测MG活化情况,MG转化为DC情况及由MG转化而来的DC表达MHC-II情况;结果:CIRI过程中MG(CD11b+)表达DC(CD11c+)表面标志;与Sham组相比,不同时间点CIRI小鼠脑组织中,活化的MG(CD11b+CD45med)显著增多(P1d<0.01,2 d、4 d、6 d组P<0.001),活化的MG转化为DC(CD11b+CD45med CD11c+)的数量显著增多(P<0.001),4 d时达到高峰,4 d组与1 d、2 d、6 d组相比显著增多(P<0.001,P<0.01,P<0.05),并且,由MG转化而来的DC表达MHC-II显著增多(P<0.001),4 d组与1 d、2 d、6 d组相比,CD11b+CD45med CD11c+MHC-II+细胞数量显著增多(P<0.001,P<0.001,P<0.05);结论:CIRI过程中MG能够转化为DC,并且,由MG转化而来的DC具有抗原递呈作用。
Objective: To investigate the transformation of microglia (MG) into dendritic cells (DCs) during the process of cerebral ischemia-reperfusion injury (CIRI). Methods: The CIRI mouse model was established by the method of thread occlusion. (CD11c +) surface expression of DC (CD11c +) during CIRI; compared with Sham group (CD11b + CD45med) were significantly increased in CIRI mice at different time points (P <0.001, P <0.001 on day 4, P <0.001) and activated MG was converted to DC + CD45med CD11c +) significantly increased (P <0.001), and peaked at 4 days. Compared with the 1st, 2nd, and 6th d, the number of CD45med CD11c + cells increased significantly (P <0.001, (P <0.001). The numbers of CD11b + CD45med CD11c + MHC-II + cells in 4d group were significantly higher than those in 1d, 2d and 6d groups (P <0.001, P <0.001, P <0.05). CONCLUSION: MG can be transformed into DC during CIRI, and DC transformed from MG has antigen presenting effect.