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目的:建立测定附马开痹片中士的宁含量的方法。方法:采用高效液相色谱法,色谱柱:CAPCELL C_(18)(150 mm×4.6mm,5μm);流动相:0.05 mol·L~(-1)磷酸二氢钠溶液-乙腈-三乙胺(80:20:0.025)(磷酸调pH至2.6);检测波长为254 nm;流速1.0 ml·min~(-1);柱温为22℃。结果:士的宁的线性范围为0.059 8～0.897 6μg,平均回收率为98.7%(RSD=1.4%,n=9)。结论:本法操作简便,快速,适用于附马开痹片中士的宁的含量测定和质量控制。
Objective: To establish a method for determining the content of strychnine in Fukai Kaili tablets. Methods: High performance liquid chromatography (HPLC) was used with a mobile phase of CAPCELL C18 (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of 0.05 mol·L -1 sodium dihydrogenphosphate solution, acetonitrile and triethylamine (80:20:25:25). The detection wavelength was 254 nm, the flow rate was 1.0 ml · min ~ (-1) and the column temperature was 22 ℃. Results: The calibration curve was linear over the range of 0.059 8-0.897 6 μg with an average recovery of 98.7% (RSD = 1.4%, n = 9). Conclusion: This method is simple, fast and suitable for the determination of the content and quality control of the strychnine in the appendix.