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采用数值分类和16S rDNAPCR-RFLP对分离自北京部分地区野大豆(Glycine soja sieb)、大豆(Glycine max)、菜豆(Phaselous vulgaris)和长萼鸡眼草(Kummerowiae stipulacea)等宿主的60株菌及10株根瘤菌参比菌株进行了研究。数值分类结果表明,在70.5%相似性水平上,所有的菌株可分为3群:群Ⅰ为未知菌群,群Ⅱ为快生和中慢生菌,群Ⅲ为慢生菌群。依据16S rDNA PCR-RFLP分析建立的树状图,在69%的相似性水平上所有的供试菌株可以分为9个系统发育分支。分支Ⅰ、Ⅴ、Ⅵ、Ⅶ、Ⅷ和Ⅸ没有参比菌,数值分类中群Ⅰ的供试菌株基本上都处于这些分支。分支Ⅱ为Sinorhizobium-Mesorhizobium-Rhizobium,分支Ⅲ为Agrobacterium分支,分支Ⅳ为Bradyrhizobium分支。
Sixty isolates of 60 isolates from hosts such as Glycine soja sieb, Glycine max, Phaselous vulgaris and Kummerowiae stipulacea isolated from parts of Beijing were analyzed by numerical classification and 16S rDNAPCR-RFLP. Rhizobia strains were studied. The results of numerical classification showed that all the isolates could be divided into three groups at 70.5% similarity level: group I was unknown group, group Ⅱ was fast and medium slow-growing bacteria, group Ⅲ was slow-growing group. Based on the 16S rDNA PCR-RFLP analysis of the established dendrogram, all tested strains were divided into 9 phylogenetic branches at 69% similarity level. Branches Ⅰ, Ⅴ, Ⅵ, Ⅶ, Ⅷ and Ⅸ had no reference bacteria, and the tested strains of group I were mostly located in these branches. Branch II is Sinorhizobium-Mesorhizobium-Rhizobium, Branch III is the Agrobacterium branch, Branch IV is the Bradyrhizobium branch.