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目的探讨神经干细胞(NSC)移植治疗新生大鼠缺氧缺血性脑损伤(HIBD)的最佳部位。方法7d 龄新生大鼠制成 HIBD 模型后随机分为 HIBD 组(12只),皮质移植组(CT,12只),海马移植组(HT,12只)和侧脑室移植组(VT,12只)。移植4周后行放射形迷宫测试学习记忆和空间辨别能力,4项感觉运动功能测试测试感觉运动功能的恢复情况。计数皮质和海马 CA1区单位面积的正常神经元。结果在放射形迷宫测试中,成绩为 HT组>VT 组>CT 组>HIBD 组[觅水时间依次为(s):48±17、51±21、58±22、89±26],各组与 HIBD 组差异均有统计学意义(均 P<0.05)。感觉运动功能测试中的成绩为 CT 组>VT 组>HT 组>HIBD 组,其中 CT 组,VT 组和 HIBD 组的差异有统计学意义(均 P<0.05)。尼氏染色示皮质区正常神经元数:CT 组>VT 组>HT 组>HIBD 组,CA1区正常神经无数:HT 组>VT组>CT 组>HIBD组[依次为(细胞/mm~2):98±12、90±8、79±9、57±10,均 P<0.05]。结论 NSC 移植到侧脑室可有效改善新生大鼠 HIBD 的全脑损伤。
Objective To investigate the optimal site of neural stem cells (NSC) transplantation for hypoxic-ischemic brain damage (HIBD) in neonatal rats. Methods 7-day-old neonatal rats were randomly divided into three groups: HIBD group (12 rats), cortical transplantation group (12 rats), hippocampus transplantation group (HT 12 rats) and lateral ventricle transplantation group ). After 4 weeks of transplantation, radial maze was used to test the ability of learning and memory and spatial discrimination, and four sensory and motor functional tests were used to test the recovery of sensory and motor functions. Normal neurons were counted per unit area of cortex and hippocampal CA1 region. Results In the radial labyrinth test, the results were HT group> VT group> CT group> HIBD group [water finding time was (48 ± 17,51 ± 21,58 ± 22,89 ± 26)] in each group And HIBD group, the differences were statistically significant (all P <0.05). The results of sensory motor function test were CT group> VT group> HT group> HIBD group, and there was significant difference between CT group, VT group and HIBD group (all P <0.05). NIH staining showed that the number of normal neurons in cortical area: CT group> VT group> HT group> HIBD group and CA1 area. There were numerous normal nerves in HT group> VT group> CT group> HIBD group [cells / mm ~ : 98 ± 12,90 ± 8,79 ± 9,57 ± 10, all P <0.05]. Conclusion Transplantation of NSC into the lateral ventricle can effectively improve the whole brain injury of HIBD in neonatal rats.