论文部分内容阅读
目的 从瘤细胞液和瘤块两种植瘤方式中建立兔肾VX2 移植癌模型 ,并分析其病理学基础和DSA影像特征 ,为介入实验研究提供大型动物肾肿瘤模型。方法 3 0只新西兰白兔随机分两组 ,每组 15只。第一组 ,B超引导下将VX2 瘤细胞 (约 5× 10 7个 /ml)经皮注入左肾下极 ;第二组 ,B超引导下将瘤组织块 (约含 10 6～ 10 8个瘤细胞 )经皮注入左肾下极。之后 ,对两组进行比较观察 :①不同方式植瘤的成活率 ;②肾内肿瘤体积变化和肿瘤生长率 ;③大体及镜下 (光镜和电镜 )瘤组织形态特征 ;④成熟模型的肾动脉DSA表现。结果 ①两组植瘤成活率分别为 4/15、11/15 ,第二组植瘤成活率最高 (Ρ <0 .0 5 ) ,其瘤体呈指数性生长 ;②病理学表明该瘤在肾组织中呈浸润式生长 ,其性状与移植于兔肝的VX2 鳞状细胞癌特征相似 ;③DSA影像示移植性肾肿瘤具有丰富的血供 ,呈实体瘤表现。结论 成功建立了兔肾VX2 移植性癌模型 ,瘤块种植方式成功率明显高于细胞液注入法 ,为实体瘤介入治疗的实验研究提供了容易复制的大型动物模型。
OBJECTIVE: To establish a model of rabbit renal VX2 carcinoma transplanted from both tumor cell and tumor mass. The pathological basis and DSA features were analyzed to provide a large animal model of renal tumor for interventional experiments. Methods Thirty New Zealand white rabbits were randomly divided into two groups (n = 15 each). In the first group, VX2 tumor cells (about 5 × 10 7 cells / ml) were percutaneously injected into the lower pole of the left kidney under the guidance of B ultrasound. In the second group, the tumor tissue mass (about 106 to 108 A tumor cells) percutaneous injection of the lower left kidney. After comparing the two groups: ① different types of tumor survival rate; ② renal tumor volume changes and tumor growth rate; ③ general and microscopic (light and electron microscopy) tumor morphological features; ④ mature model of kidney Arterial DSA performance. Results ① The survival rates of the two groups were 4/15, 1/15, respectively. The survival rate of the second group was the highest (P <0.05), and the tumor grew exponentially. ② The pathology showed that the tumor was The infiltrative growth in kidney tissue was similar to that of VX2 squamous cell carcinoma transplanted in rabbit liver. (3) The DSA images showed that the transplanted renal tumors had rich blood supply and showed solid tumors. Conclusion The rabbit VX2 transplanted carcinoma model was successfully established. The success rate of tumor implantation was significantly higher than that of cytokine injection. It provided a large animal model for the experimental study of solid tumor interventional therapy.