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为了探讨喉鳞癌中 p5 3基因与肿瘤临床分期、病理分化程度及淋巴结转移的关系 ,应用 PCR- SS-CP银染系统检测 6 0例喉鳞癌新鲜组织中抑癌基因 p5 3基因第 7外显子的突变情况。结果 :p5 3基因第 7外显子突变 2 5例 ,突变率为 41.7% .临床 ～ 期突变率为 2 6 .9% (7/ 2 6 ) , ～ 期突变率为 5 2 .9% (18/ 34) ;高、中、低分化程度分布为 2 3.5 % (4 / 17)、37.5 % (9/ 2 4)、6 3.2 % (12 / 19) ;无淋巴结转移者为 2 8.1% (9/ 32 ) ,有淋巴结转移者为5 7.1% (16 / 2 8) ;差异均有显著意义 (P<0 .0 5 )。结论 :提示 p5 3基因第 7外显子突变与喉鳞癌临床分期、病理分化程度及肿瘤颈淋巴结转移密切相关。
To investigate the relationship between p5 3 gene and the clinical stage, pathological differentiation and lymph node metastasis in laryngeal squamous cell carcinoma, PCR-SS-CP silver staining was used to detect the expression of p5 3 gene Exon mutation. Results: There were 25 mutations in exon 7 of p5 3 gene, with a mutation rate of 41.7%. The clinical-stage mutation rate was 26.9% (7/2 6), and the ~ stage mutation rate was 52.9% 18.5% (4/17), 37.5% (9/2 4), and 6 3.2% (12/19) respectively. There was no significant difference between the two groups 9/32), and those with lymph node metastasis were 51.1% (16/28). There were significant differences between the two groups (P <0.05). Conclusion: It is suggested that the mutation of exon 7 of p5 3 gene is closely related to the clinical stage, pathological differentiation and cervical lymph node metastasis of LSCC.