自60年代起,多种研究表明,每种生物大分子内都有一恒定量的不冻水。例如,多种方法测定溶菌酶的不冻水量R_(nf)为0.33±0.03g(水)/g(蛋白)。通常认为这部分水以氢键结合于大分子,维系着其结构和生物功能。故R_(nf)广泛用作刻画大分子同水相互作用的重要特征参量,并依此提出有关大分子水合的较系统的假说和分子模型。近来我们对多种生物大 Since the 1960s, various studies have shown that there is a constant amount of unfrozen water in each biological macromolecule. For example, the amount of unfrozen water Rf (nf) determined by various methods for lysozyme is 0.33 ± 0.03 g (water) / g (protein). It is generally believed that this part of the water hydrogen bonds to the macromolecules, maintaining its structure and biological function. Therefore R_ (nf) is widely used as an important characteristic parameter to characterize the water-water interaction of macromolecules. Based on this, a systematic hypothesis and molecular model for macromolecule hydration are proposed. Recently we have a large variety of creatures