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目的 研究大鼠局灶性脑缺血再灌注后神经细胞凋亡和Bcl-2蛋白表达的变化及银杏叶提取物对其表达的影响。方法 制造大鼠大脑中动脉缺血再灌注模型。40只Wistar雄性大鼠被随机分为A假手术组、B缺血组、C小剂量治疗组和D大剂量治疗组。于术前30min及术后1h分别给予银杏叶提取物20mg/kg和40mg/kg腹腔内注射。应用TTC染色及HE染色观察梗死体积及缺血坏死程度,应用免疫组化染色及POD法检测Bcl-2蛋白表达及凋亡细胞数。结果 C、D两组梗死体积明显小于B组(P<0.01),D组梗死体积小于C组(P<0.05);C、D两组凋亡细胞数明显少于B组(P<0.01),D组凋亡细胞数少于C组(P<0.05);C、D两组Bcl-2蛋白表达明显高于B组(P<0.01),D组Bcl-2蛋白表达高于C组(P<0.05)。结论 银杏叶提取物可通过上调Bcl-2蛋白表达,减少神经细胞凋亡,对脑缺血再灌注损伤起保护作用,疗效与剂量有关。
Objective To investigate the changes of neuronal apoptosis and Bcl-2 protein expression after focal cerebral ischemia-reperfusion in rats and the effect of Ginkgo biloba extract on its expression. Methods Rat middle cerebral artery occlusion (MCAO) model was established. Forty Wistar male rats were randomly divided into sham operation group A, B ischemia group, C low dose treatment group and D high dose treatment group. Ginkgo biloba extract 20mg / kg and 40mg / kg were injected intraperitoneally 30min before operation and 1h after operation respectively. The infarct volume and the degree of ischemic necrosis were observed by TTC staining and HE staining. The expression of Bcl-2 protein and the number of apoptotic cells were detected by immunohistochemistry and POD assay. Results The infarct volume in groups C and D were significantly smaller than that in group B (P <0.01), and the infarct volume in group D was less than that in group C (P <0.05). The numbers of apoptotic cells in groups C and D were significantly less than those in group B (P <0.01) , The number of apoptotic cells in group D was less than that in group C (P <0.05); the expression of Bcl-2 protein in group C and group D was significantly higher than that in group B (P <0.01) P <0.05). Conclusion Ginkgo biloba extract can protect the cerebral ischemia-reperfusion injury by up-regulating the expression of Bcl-2 protein, reducing the apoptosis of nerve cells, and the therapeutic effect is dose-dependent.