目的:评价孕期大鼠全氟辛烷磺酸(PFOS)染毒后对子代大鼠体重、脏器系数、睁眼和张耳等发育状况的影响。方法:将120只雌鼠、40只雄鼠分别按体重随机各分为4组,分别为对照组、低剂量组、中剂量组和高剂量组。适应性喂养1周后雌雄鼠合笼,1周后处死雄鼠。合笼第1天开始到整个孕期结束分别对低剂量组、中剂量组和高剂量组用不同剂量的PFOS每天灌胃染毒大鼠,剂量依次为PFOS 0.4 mg·kg-1·d-1、0.8 mg·kg-1·d-1和1.6 mg·kg-1·d-1,对照组不给予。哺乳期停止染毒,哺乳结束时(哺乳1个月)髂静脉取血后处死所有亲代大鼠及2只子代大鼠。处死后取肝脏、胸腺、睾丸、卵巢和子宫,称上述组织的重量并计算脏器系数。喂养过程中记录哺乳期子代大鼠1、4、7、14、21、28天时的体重,观察大鼠的睁眼和张耳时间。1个月后每周记录剩余大鼠的体重。结果:出生前7天各组的平均体重无差异,而7天后对照组的平均体重低于3个实验组。对照组睁眼时间及张耳时间分别主要集中在出生后的第14天与第16天,较3个实验组的睁眼及张耳时间早,差异有统计学意义(P<0.025 0)。雌鼠的各脏器系数无差异,对照组雄鼠睾丸的脏器系数低于中剂量组及高剂量组,差异有统计学意义(P<0.05)。而其他脏器系数无差异。结论:孕期PFOS染毒会对子代大鼠的体重、脏器系数、睁眼和张耳等发育状况造成一定的影响。 OBJECTIVE: To evaluate the effects of PFOS exposure on body weight, organ coefficient, eye opening and ear development during pregnancy in rats. Methods: 120 female rats and 40 male rats were randomly divided into 4 groups according to body weight, which were control group, low dose group, middle dose group and high dose group respectively. After 1 week of adaptive feeding, male and female rats were caged, and male rats were sacrificed one week later. Rats in the low-dose group, medium-dose group and high-dose group were given gavage daily with different doses of PFOS at the dose of 0.4 mg · kg-1 · d-1 , 0.8 mg · kg-1 · d-1 and 1.6 mg · kg-1 · d-1 in the control group. Breastfeeding was stopped and all the offspring rats and 2 offspring rats were killed after the end of lactation (breastfeeding for 1 month). After sacrifice to take liver, thymus, testis, ovary and uterus, said the weight of the above tissue and calculate organ coefficients. During feeding, the body weights of suckling offspring rats at 1, 4, 7, 14, 21 and 28 days were recorded. The rats’ eyes were opened and their ears were exposed. The remaining rats were recorded weekly after 1 month. RESULTS: There was no difference in mean body weight between groups 7 days before birth, while the average body weight of the control group after 7 days was lower than that of the 3 experimental groups. The eyes of the control group and the time of opening of the ears were mainly on the 14th and 16th days after birth, respectively, which were earlier than those of the three experimental groups (P <0.025 0). There was no significant difference in the organ coefficient of female rats, and the testis organ coefficient of male rats in control group was lower than that of middle dose group and high dose group (P <0.05). The other organ coefficient no difference. Conclusions: PFOS exposure during pregnancy may affect the body weight, organ coefficient, eyes open and ears of the offspring.