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目的评估PCR-反向点杂交法在HBV耐药突变基因检测中的临床价值和应用前景。方法采用PCR-反向点杂交法的检测技术对488例乙肝患者的血清进行HBV多个耐药位点的检测,并同时进行病毒的基因分型。结果 488例慢性乙肝患者的HBV,检出C型286例(58.61%),B型164例(33.61%),B+C混合型23例(4.71%),D型15例(3.07%);89例发生不同位点变异,变异率为18.24%。结论 PCR-反向点杂交法是一种实用、直接、操作简单快捷,适合临床推广使用的HBV耐药突变基因的检测方法。
Objective To evaluate the clinical value and application of PCR-reverse dot blot in the detection of HBV resistance mutations. Methods PCR-reverse dot hybridization was used to detect the HBV multi-drug resistant loci in 488 patients with hepatitis B and the genotypes were also detected. Results HBV was detected in 488 patients with chronic hepatitis B, 286 cases (58.61%) were C type, 164 cases (33.61%) were B type, 23 cases (4.71%) were B + C mixed type and 15 cases (3.07%) were D type. 89 cases of different site mutation, mutation rate was 18.24%. Conclusion PCR-reverse dot hybridization is a practical, direct, simple and efficient method for detection of HBV resistance mutations in clinical practice.