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目的:探讨P53和ATRX蛋白在IDH1突变型与野生型胶质母细胞瘤的表达相关性及生物学意义。方法:收集广东同江医院病理科行手术切除胶质母细胞瘤后组织蜡块标本共62例(其中经免疫组织化学检测IDH1突变型胶质母细胞瘤12例,IDH1野生型胶质母细胞瘤50例),检测P53和ATRX蛋白在这两种不同基因分型胶质母细胞瘤中的表达情况及相关性,并分析其与预后的关系。结果:P53蛋白在IDH1突变型与野生型胶质母细胞瘤的表达率分别为75.00%(9/12)和30.00%(15/50),ATRX蛋白的表达率分别为16.67%(2/12)及94.00%(47/50),P53和ATRX蛋白在IDH1两种基因分型的胶质母细胞瘤的表达差异均有统计学意义(均P<0.01)。P53及ATRX的表达呈负相关(r=-0.404,P<0.01)。Cox多元回归分析结果显示IDH1基因分型、年龄及性别是影响胶质母细胞瘤(glioblastoma,GBM)生存期的危险因素(P<0.01)。结论:大部分的IDH1野生型的GBM的患者缺乏P53基因及ATRX基因的突变。IDH1野生型GBM是显著影响GBM生存期的危险因素,可以作为临床对成年GBM患者预后判断的重要指标之一。
Objective: To investigate the relationship and biological significance of P53 and ATRX protein in IDH1 mutant and wild type glioblastoma. Methods: Totally 62 cases of glioblastoma specimens were collected from the Department of Pathology of Tongjiang Hospital of Guangdong Province (including 12 cases of IDH1 mutant glioblastoma detected by immunohistochemical staining, IDH1 wild type glioblastoma Tumor in 50 cases). The expression and correlation of P53 and ATRX protein in these two different genotypes of glioblastoma were detected and their relationship with prognosis was analyzed. Results: The expression rates of P53 protein in IDH1 mutant and wild glioblastoma were 75.00% (9/12) and 30.00% (15/50), respectively. The expression rates of ATRX protein were 16.67% (2/12 ) And 94.00% (47/50), respectively. The expression of P53 and ATRX protein in both genotypes of IDH1 was significantly different (all P <0.01). P53 and ATRX were negatively correlated (r = -0.404, P <0.01). Cox multivariate regression analysis showed that IDH1 genotyping, age and gender were risk factors for the survival of glioblastoma (GBM) (P <0.01). Conclusions: Most patients with IDH1 wild-type GBM lack mutations in the P53 and ATRX genes. IDH1 wild-type GBM is a risk factor that significantly affects the survival of GBM, which can be used as one of the important clinical indicators of prognosis in adult GBM.