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目的 研究胃腔内促性腺激素释放激素 (GnRH)类似物对大鼠消化道胃泌素免疫阳性细胞及血液、胃液中胃泌素含量的影响。 方法 应用免疫组织化学和酶联免疫分析 (ELISA)方法分别检测大鼠消化道内胃泌素阳性细胞的密度及血液、胃液中胃泌素的含量。 结果 胃腔注射GnRH类似物后 ,胃壁内单位面积胃泌素阳性细胞数为 19 6 0± 3 6 3,与对照组 10 30± 2 4 1相比 (P <0 0 1) ;而十二指肠阳性细胞数为 18 0 0± 2 31,与对照组 9 0 0± 2 0 5相比 (P <0 0 1)。血液中胃泌素ELISA检测的A值为 0 5 5± 0 0 83,与对照组 0 2 3± 0 0 39相比 (P <0 0 1) ;胃液中为 0 5 2± 0 0 83,与对照组 0 30± 0 0 31相比 (P <0 0 1)。 结论 外分泌的GnRH对大鼠消化道中胃泌素的合成与分泌均起显著的促进作用。
Objective To investigate the effect of GnRH analogues on gastrin-positive cells and gastrin in blood and gastric juice of rats. Methods The density of gastrin-positive cells in the digestive tract and the content of gastrin in blood and gastric juice were detected by immunohistochemistry and enzyme-linked immunosorbent assay (ELISA). Results After intragastric injection of GnRH analogues, the number of gastrin-positive cells per unit area in the stomach wall was 19 6 0 ± 3 6 3, which was significantly higher than that of the control group 10 30 ± 2 4 1 (P 0 01) The number of nosocomial positive cells was 18 0 0 ± 2 31, which was significantly higher than that of the control group 90 0 ± 20 5 (P 0 01). The A value of gastrin ELISA in blood was 0 5 5 ± 0 0 83, which was significantly lower than that of control group 0 2 3 ± 0 0 39 (P 0 01), 0 5 2 ± 0 0 83 in gastric juice, Compared with control group 0 30 ± 0 0 31 (P 0 01). Conclusion Exocrine GnRH plays a significant role in the synthesis and secretion of gastrin in the digestive tract of rats.