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次甲基兰水溶液的最大吸收波长为663.5nm。肝素与次甲基兰水溶液反应后,其吸光度A的降低与肝素浓度(效价)成正比。溶液颜色稳定,采用比较法测定。肝素浓度在0.01-).05mg/25ml范围内服从朗伯比尔定律。平均回收率99.73％。变异系数0.53％。该法可用于肝素钠注射液的效价测定。
The maximum absorption wavelength of the methylene blue aqueous solution is 663.5nm. After the reaction of heparin with methine blue solution, the decrease of absorbance A is proportional to the heparin concentration (titer). Solution color stability, using comparative method. Heparin concentration in the 0.01 -). 05mg / 25ml obey Lambertil law. The average recovery rate of 99.73%. The coefficient of variation is 0.53%. This method can be used to determine the titer of heparin sodium injection.