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采用猪伪狂犬病毒(PRV)ZJ01株纯化病毒免疫BALB/c小鼠,利用融合细胞技术和间接ELISA抗体筛选技术,制备并获得2株能稳定分泌抗PRV单克隆抗体的杂交瘤细胞株2B3和5C10,其中,2B3单抗为Ig G2a亚类,5C10为Ig G1亚类,轻链均为κ链。间接免疫荧光检测结果表明,2株单克隆抗体均能与PRV发生特异性反应。Western-blot结果表明,2B3单抗针对PRV g C蛋白,5C10单抗针对PRV g E蛋白。本研究为建立快速检测伪狂犬病毒感染的免疫学方法奠定了基础。
BALB / c mice were immunized with PRV strain ZJ01 and purified by fusion cell technology and indirect ELISA antibody screening to prepare and obtain two hybridoma cell lines 2B3 and 2B3 which can stably secrete anti-PRV monoclonal antibody 5C10, in which, 2B3 monoclonal antibody is Ig G2a subclass, 5C10 is Ig G1 subclass, light chain is κ chain. Indirect immunofluorescence assay showed that both monoclonal antibodies reacted specifically with PRV. Western-blot results showed that 2B3 mAb was against PRV gC protein and 5C10 mAb against PRV gE protein. This study lays the foundation for establishing an immunological method for rapid detection of PRV infection.