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目的：了解正常藏族人群载脂蛋白Ｂ（ａｐｏＢ） 基因多态性及其血脂特点。方法：随机选取１００ 例正常成年藏族受试者，男女各半，年龄１８ ～６０ 岁。测定其血清甘油三脂（ＴＧ） 、总胆固醇（ＴＣ） 、低密度脂蛋白胆固醇（ＬＤＬ－ Ｃ） 、高密度脂蛋白胆固醇（ ＨＤＬ－ Ｃ） 、ａｐｏＡＩ、ａｐｏＢ 和脂蛋白（ａ）［Ｌｐ（ａ）］ 的浓度；并采用ＰＣＲ 方法检测其ａｐｏＢ 基因信号肽插入／缺失（ｉｎｓ／ｄｅｌ） ＤＮＡ 片段的多态性，计算各等位基因的分布频率。结果：藏族人群中以ｉｎｓ 等位基因分布为主（０－７４５） ，ｄｅｌ等位基因频率占０－２５５ ，两种等位基因分布无明显的性别差异。组成的三种基因型分别为ｉｎｓ／ｉｎｓ ５６ 例、ｉｎｓ／ｄｅｌ ３７ 例、ｄｅｌ／ｄｅｌ ７ 例。与汉族人群比较，藏族ｉｎｓ／ｄｅｌ 等位基因的频率与其相近，但ｄｅｌ 等位基因频率显著低于西方高加索人种（０－３４０ ，Ｐ＜ ０－０５） 。藏族人群中男女各年龄段（１８ ～３９ 岁、４０ ～６０ 岁） 的各项血脂指标变化不大，但ＴＣ、ＬＤＬ－ Ｃ 和ａｐｏＢ的含量显著低于汉族和西方人群，Ｌｐ（ａ） 的水平与后者接近。ｄｅｌ 等位基因的个体其ＬＤＬ－ Ｃ 的浓度高于ｉｎｓ 者，特别是
Objective: To understand the polymorphism of apolipoprotein B (apoB) gene and its lipid profile in normal Tibetans. Methods: 100 normal adult Tibetan subjects were randomly selected, half male and half female aged 18-60 years. Serum triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apoAI, apoB and lipoprotein (a) [Lp a)]. The polymorphism of insp-del DNA fragment of apoB gene was detected by PCR and the distribution frequency of each allele was calculated. Results: The distribution of ins alleles was mainly from Tibetan population (0-745). The frequency of del alleles was 0-255. There was no significant gender difference in the two alleles. Composition of the three genotypes were ins / ins 56 cases, ins / del 37 cases, del / del 7 cases. Compared with the Han population, the frequency of Tibetan ins / del allele was similar to that of the Han population, but the frequency of the del allele was significantly lower than that of western Caucasians (0-340, P <0-05). The blood lipid level of Tibetan men and women in all age groups (18-39 years old, 40-60 years old) had little change, but the contents of TC, LDL-C and apoB were significantly lower than those in Lp (a) Close to the latter level. Individuals with the del allele have higher concentrations of LDL-C than ins, in particular