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目的:探讨氧化苦参碱(OMT)对D-半乳糖致小鼠衰老的影响。方法:健康昆明种小鼠,随机分为正常对照组、模型组以及氧化苦参碱低(5 mg/kg)、中(10 mg/kg)、高(20 mg/kg)剂量组,除正常对照组外,各组动物给予D-半乳糖0.1 g/kg造模,造模1 h后给药。各组连续腹腔给药30 d后进行跳台实验及Morris水迷宫实验以测定小鼠的学习记忆能力。待行为学指标测完后,将各组小鼠眼静脉丛取血1～2 mL,离心取血清,测定SOD活性以及MDA含量。结果:在水迷宫实验中,模型组较正常对照组小鼠到达终点平台的潜伏期显著延长(P<0.01),穿越平台次数显著减少(P<0.01),而各给药组较模型组小鼠到达终点平台的潜伏期显著缩短(P<0.05或P<0.01),穿越平台的次数显著增加(P<0.05或P<0.01);在小鼠跳台实验中,OMT各剂量均显示出延长平台潜伏期、减少错误次数的趋势,但差异无统计学意义(P>0.05);在血清生化指标检测中,模型组SOD活性显著低于正常对照组(P<0.01),而氧化苦参碱各剂量组血清SOD活性均较模型组显著增高(P<0.05或P<0.01);模型组血清MDA含量显著高于正常对照组(P<0.01),而氧化苦参碱各剂量组血清MDA含量均显著低于模型组(P<0.05或P<0.01)。结论:氧化苦参碱能够增加D-半乳糖致小鼠衰老的学习记忆能力,该作用与其抗氧自由基,减少脂质过氧化有关。
Objective: To investigate the effect of oxymatrine (OMT) on the aging of mice induced by D-galactose. Methods: Healthy Kunming mice were randomly divided into normal control group, model group and oxymatrine low (5 mg / kg), medium (10 mg / kg) and high (20 mg / kg) The control group, each group of animals given D-galactose 0.1 g / kg modeling, modeling 1 h after administration. After 30 days of continuous intraperitoneal administration in each group, the platform test and the Morris water maze test were performed to determine the learning and memory abilities of mice. To be behavioral indicators after the test, the mice were ophthalmic plexus blood 1-2 mL, centrifuged serum was measured SOD activity and MDA content. Results: In the water maze test, the latency of the model group reaching the terminal platform was significantly longer than that of the normal control group (P <0.01), and the number of crossing the platform was significantly decreased (P <0.01) The latency to reach the end platform was significantly shorter (P <0.05 or P <0.01) and the number of crossing platforms significantly increased (P <0.05 or P <0.01) Reduce the number of errors, but the difference was not statistically significant (P> 0.05); in serum biochemical detection, SOD activity in model group was significantly lower than that in normal control group (P <0.01) (P <0.05 or P <0.01). The level of serum MDA in the model group was significantly higher than that in the normal control group (P <0.01), while the level of MDA in each dose of oxymatrine was significantly lower than that in the model group Model group (P <0.05 or P <0.01). Conclusion: Oxymatrine can increase the learning and memory ability of aging mice induced by D-galactose, which is related to its antioxidant free radicals and lipid peroxidation.