目的 :研究环境有毒物质镍和亚砷酸盐对辐射引起的DNA双链断裂 (dsb)修复的影响。方法 :取 3× 10 5 中国地鼠卵细胞在 6 0 mm组织培养皿中 (5 m L Ham′s F- 12培养液 )于 37℃培养 4d,另取 1.5× 10 5细胞在 35 m m组织培养皿中加 2 m L培养液和 0 .74k Bq/ m L1 4 C-胸苷培? OBJECTIVE: To study the effect of environmental toxic substances, nickel and arsenite, on radiation-induced DNA double-strand break (dsb) repair. Methods: 3 × 10 5 Chinese hamster ovary cells were cultured in 600 mm tissue culture dishes (5 m L Ham’s F-12 medium) for 4 days at 37 ℃. Another 1.5 × 10 5 cells were cultured in 35 mm tissue culture Add 2 mL culture medium and 0.74k Bq / mL L1 4 C-thymidine culture medium