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目的:探讨腺病毒介导的转化生长因子β-1(TGF-β1)对结肠癌细胞凋亡的诱导作用。方法:结肠癌细胞系HCT116细胞培养后分实验组及对照组,实验组以腺病毒为载体将TGF-β1转染,逆转录聚合酶链反应(RT-PCR)以及免疫组织化学检测其m RNA及蛋白的表达,MTT法检测细胞生长抑制率,流式细胞仪检测细胞凋亡的情况。结果:实验组的TGF-β1 mRNA以及蛋白的表达明显增强;实验组细胞的吸光度波动较小,在低值区相对稳定,各时相点无明显变化,24 h的细胞增殖抑制率为50%,其后在70%-80%之间;对照组细胞吸光度显著升高,与实验组各时相点比较,差异有统计学意义(P<0.01)。实验组24 h、36 h、48 h、60 h、72 h的凋亡率分别为(7.55±0.03)%、(8.53±0.11)%、(13.47±0.23)%、(15.51±0.26)%、(16.59±0.26)%,与对照组细胞各时相点比较差异有统计学意义(P<0.01)。结论:TGF-β1能够显著地抑制HCT 116细胞的增值及诱导其凋亡。
Objective: To investigate the effect of adenovirus-mediated transforming growth factor-β1 (TGF-β1) on the apoptosis of colon cancer cells. Methods: The colon cancer cell line HCT116 cells were divided into experimental group and control group. TGF-β1 transfection, reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to detect m RNA And protein expression. MTT assay was used to detect the rate of cell growth inhibition and flow cytometry to detect apoptosis. Results: The expression of TGF-β1 mRNA and protein in the experimental group was significantly increased. The absorbance of the experimental group was less fluctuated, and was relatively stable in the low-value area, with no significant change at each time point. The cell proliferation inhibition rate was 50% , And then between 70% -80%. The absorbance of the control group increased significantly, which was significantly different from that of the experimental group (P <0.01). The apoptotic rates in experimental group at 24 h, 36 h, 48 h, 60 h and 72 h were (7.55 ± 0.03)%, (8.53 ± 0.11)%, (13.47 ± 0.23)% and (15.51 ± 0.26)%, (16.59 ± 0.26)%, which was significantly different from that of the control group (P <0.01). Conclusion: TGF-β1 can significantly inhibit the proliferation of HCT 116 cells and induce their apoptosis.