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疫霉根腐病能使大豆感病品种减产5％～10％, 并造成品质降低.利用转基因技术培育抗病品种是解决该问题的有效途径之一.对转hrpZpsta和chi双价广谱抗病基因株系T2003-23-11、T2004-28-321和T2004-30-384进行分子检测并鉴定其抗疫霉根腐病能力.Southern Blot结果表明, hrpZpsta和chi双价基因已整合在株系的核基因组中而且稳定遗传到T4代;qRT-PCR结果表明, hrpZpsta和chi双价基因在株系的不同组织中的相对表达量均高于非转基因对照.利用下胚轴侵染法对3个株系进行抗疫霉根腐病鉴定结果表明, 3个株系抗病级别均为高抗, 非转基因对照的抗病级别为中抗, 表明转基因株系的抗病能力得到提高.“,”Phytophthora root rot can make yield of sensitive soybean cultivar decrease by 5％～10％ and quality decrease, so cultivating disease-resistant varieties by transgenic technology becomes the effective way of solving the problem. Molecular detection of strains T2003-23-11, T2004-28-321 and T2004-30-384 was conducted and their disease-resistant ability was identified with two broad spectrum disease-resistant genes hrpZpsta and chi. Southern Blot results demonstrate hrpZpsta and chi genes have integrated in the nuclear genome and stably inherited to T4 generation. qRT-PCR results demonstrate expression in different tissues of strains are higher than that of the non-transgenic control. Phytophthora root rot of three strains was identified by hypocotyl infection method, and the results show that resistance level of the three strains is high, and resistance level of non-transgenic control is moderate, showing that disease resistance of transgenic strains is improved.