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为了从细胞凋亡角度探讨 4-〔4″- ( 2″,2″,6″,6″-四甲基 - 1″-哌啶氮氧自由基 )氨基〕- 4′-去甲表鬼臼毒素 ( GP7)抗肿瘤作用的机理 ,用活细胞计数法及MTT比色法观察了 GP7对 NB4细胞的生长抑制作用 ;用光学显微镜及电子显微镜观察了凋亡细胞的形态学变化 ;用琼脂糖凝胶电泳观察了 DNA的梯形改变 .结果发现 :GP70 .1 8- 1 8μmol· L-1能显著抑制 NB4细胞的生长增殖 ;GP7可明显诱导NB4细胞凋亡的形态学变化及 DNA梯形带 .凋亡率随处理时间延长而增高 . 9μmol· L-1处理 NB4细胞 48h凋亡率达到最高峰 ,为 45.0± 3.0 ) % .延长处理时间至 72 h,凋亡率降至 ( 2 6.7± 1 .5) % .凋亡率与药物浓度的对数呈正相关 ( r=0 .938,P<0 .0 5) .结果表明 GP7具有诱导 NB4细胞凋亡作用 .
In order to investigate the effects of 4- [4 “- (2”, 2 “, 6”, 6 “-tetramethyl- 1” -piperidine nitroxide) The anti-tumor effect of podophyllotoxin (GP7) was observed. The growth inhibition of GP4 on NB4 cells was observed by viable cell counting and MTT colorimetric assay. Morphological changes of apoptotic cells were observed by light microscopy and electron microscopy. Glucose gel electrophoresis was used to observe the trapezoidal changes of DNA. The results showed that: GP70. 1 8- 1 8μmol · L-1 can significantly inhibit the proliferation of NB4 cells; GP7 can obviously induce apoptosis of NB4 cells and DNA ladder .Apoptosis rate increased with the prolongation of treatment time.Apoptosis rate of NB4 cells treated with 9μmol · L-1 reached the peak at 48h, which was 45.0 ± 3.0% 1.5%). The apoptotic rate was positively correlated with the logarithm of drug concentration (r = 0. 938, P <0.05). The results showed that GP7 could induce the apoptosis of NB4 cells.