本文用HPLC测定、比较了5种不同来源的高山红景天材料中有效成分—红景天甙及其甙无酪醇的含量,并建立了高山红景天有效成分分析的样品制备方法,结果表明:固体培养、液体培养及反应器培养的高山红景天组织中均含有上述两种有效成分.其中固体培养的材料中有效成分的含量最高.三种提取方法比较表明,用索氏提取器连续回流提取明显优于冷浸提取和批式回流提取. In this paper, HPLC was used to determine the content of salidroside and its non-tyrosol, which are the active ingredients in Rhodiola sachalinensis from five different sources, and the method for preparing the active ingredient of Rhodiola sachalinensis was established. The results The results showed that these two active ingredients were contained in solid culture, liquid culture and Rhodiola sachalinensis, both of which contained the highest content of active ingredients in solid culture materials.The comparison of the three extraction methods showed that using Soxhlet extractor Continuous reflux extraction was significantly better than cold extraction and batch extraction.