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目的:探讨人基因组rs2200733位点的多态性与中国汉族中青年高血压患者并发房颤发生的关联性。方法:根据入选标准,选择中国汉族中青年高血压并发房颤患者208例(房颤组)和不伴房颤的高血压患者405例(对照组)。采集患者外周血,提取基因组DNA,采用聚合酶链反应-限制性酶切片段长度多态性技术检测rs2200733位点的基因型和等位基因分布。结果:rs2200733位点存在多态性,分别为TT、TC和CC型,其基因型频率在房颤组和对照组分别为58.1%、33.7%、8.2%和72.9%、25.9%、1.2%。基因型分布有显著性差异(P<0.001),房颤组TC和CC基因型频率明显高于对照组(P<0.001)。Logistic多因素回归分析显示rs2200733位点多态性与房颤显著相关(OR=3.143,95%CI:1.442-6.581)。结论:rs2200733位点多态性与中国汉族中青年高血压患者的房颤发生存在相关性,TC和CC基因型可能是房颤发生的一个遗传易感因素。
Objective: To investigate the association between human genomic rs2200733 polymorphism and the occurrence of atrial fibrillation in middle-aged and young Chinese hypertensive patients. Methods: According to the inclusion criteria, 208 Chinese hypertensive patients with atrial fibrillation (AF group) and 405 hypertensive patients without AF (control group) were selected. The peripheral blood of patients was collected and genomic DNA was extracted. The genotype and allele distribution of rs2200733 locus were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The rs2200733 polymorphism was found in TT, TC and CC genotypes. The genotype frequency of rs2200733 was 58.1%, 33.7%, 8.2% and 72.9%, 25.9% and 1.2% in the AF group and the control group, respectively. (P <0.001). The frequencies of TC and CC genotypes in AF group were significantly higher than those in control group (P <0.001). Logistic regression analysis showed that rs2200733 polymorphism was significantly associated with atrial fibrillation (OR = 3.143, 95% CI: 1.442-6.581). Conclusion: The rs2200733 polymorphism is associated with atrial fibrillation in middle-aged and young Chinese hypertensive patients in Han nationality. TC and CC genotypes may be a genetic predisposition to AF.