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目的观察胰岛素对脂多糖诱导的急性肺损伤大鼠的影响。方法 SD大鼠72只随机分为3组,模型组和胰岛素组予内毒素5mg经颈静脉插管注射,复制急性肺损伤模型;空白对照组给予生理盐水1mL·kg~(-1)。2h后,空白对照组、模型组经微量泵泵入生理盐水1mL·kg~(-1)·h~(-1),胰岛素组泵入含0.5kU·L~(-1)胰岛素的生理盐水溶液1mL·kg~(-1)·h~(-1)。以微量泵泵入开始为0点,分别于0、30min,1、2h抽取静脉血测肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)水平,并观察肺组织病理学形态,计算肺湿干重比。结果各观察时点模型组和胰岛素组大鼠血清IL-1β、TNF-α水平和肺组织病理评分均高于空白对照组,差异显著(P<0.05)。微量泵泵入1、2h,模型组大鼠肺湿干重比显著高于空白对照组(P<0.05)。胰岛素治疗后1、2h,胰岛素组大鼠血清IL-1β、TNF-α水平和肺组织病理评分、肺湿干重比均低于模型组,差异有显著意义(P<0.05)。结论胰岛素可以抑制脂多糖诱导的急性肺损伤大鼠的炎症反应并减轻肺水肿,对急性肺损伤大鼠有一定的保护和治疗作用。
Objective To observe the effect of insulin on lipopolysaccharide-induced acute lung injury in rats. Methods Seventy-two SD rats were randomly divided into three groups. The model group and the insulin group were injected endotoxin 5mg through the jugular vein, and the model of acute lung injury was induced. The blank control group was given normal saline (1mL · kg -1). After 2h, the blank control group and model group were infused with normal saline (1mL · kg -1 h -1) by micro pump and insulin group (0.5kU · L -1 insulin) Aqueous solution 1mL · kg -1 (-1) h -1. The level of TNF-α and IL-1β in venous blood was measured at 0, 30, Morphology, lung wet to dry weight ratio calculation. Results Serum levels of IL-1β, TNF-α and lung histopathological score in model group and insulin group were significantly higher than those in blank control group at each observation point (P <0.05). When the pump was pumped for 1 and 2 hours, the lung wet / dry weight ratio in the model group was significantly higher than that in the blank control group (P <0.05). The levels of IL-1β, TNF-α, lung histopathological score and lung wet / dry weight ratio in insulin group were significantly lower than those in model group at 1 and 2 h after insulin treatment (P <0.05). Conclusion Insulin can inhibit lipopolysaccharide-induced acute lung injury in rats with inflammatory response and reduce pulmonary edema, acute lung injury in rats have a protective and therapeutic effect.