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用高效液相色谱解析Ｌａ（Ⅲ）分别与单个脱氧核糖核苷酸反应、与碱基对互补的５′－ｄＡＭＰ和５′－ｄＴＭＰ、５′－ｄＣＭＰ和５′－ｄＧＭＰ反应、与４种脱氧核糖核苷酸共存体系反应的产物．从色谱峰的变化，证明了在ｐＨ６．５、室温条件下，Ｌａ（Ⅲ）可与单个的５′－ｄＧＭＰ作用；不与单个的５′－ｄＡＭＰ、５′－ｄＴＭＰ、５′－ｄＣＭＰ相作用，但在５′－ｄＡＭＰ和５′－ｄＴＭＰ共存时，Ｌａ（Ⅲ）使５′－ｄＡＭＰ和５′－ｄＴＭＰ的色谱峰面积大大减小并有新峰产生；当四种脱氧核糖核苷酸同时存在时除５′－ｄＣＭＰ峰不变外，５′－ｄＡＭＰ、５′－ｄＴＭＰ和５′－ｄＧＭＰ的峰面积都明显减小．这一结果表明：Ｌａ（Ⅲ）可与５′－ｄＡＭＰ和５′－ｄＴＭＰ相互交联，从而为稀土离子与ＤＮＡ双链进行链间交联的推测提供了有力的证据．
High performance liquid chromatography (HPLC) was used to analyze the reaction of La (Ⅲ) with single deoxyribonucleotide, 5’-dAMP and 5’-dTMP, 5’-dCMP and 5’-dGMP, Deoxyribonucleotide coexistence system reaction product. From the change of chromatographic peak, La (III) was proved to be able to interact with single 5’-dGMP at pH6.5 and room temperature, but not with single 5’-dAMP, 5’-dTMP, 5’-dCMP However, when Co-presence of 5’-dAMP and 5’-dTMP, La (Ⅲ) greatly reduced the peak area of 5’-dAMP and 5’-dTMP and produced a new peak. When four kinds of deoxyribonucleosides In the presence of acid, the peak areas of 5’-dAMP, 5’-dTMP and 5’-dGMP were significantly reduced except for the 5’-dCMP peak. This result indicates that La (III) can cross-link with 5’-dAMP and 5’-dTMP to provide strong evidence for the speculation that interchain cross-linking of rare earth ions and DNA duplexes.