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寻找一种符合患者治疗需求的干细胞获取模式,从而进一步推动神经干细胞广泛应用于临床。本实验采用志愿者、患者脑脊液体外诱导异体脐血源间充质干细胞(UMSCs)和患者骨髓源间充质干细胞(BMSCs)分化为神经干细胞,并采用形态学、流式细胞分析、免疫组化、荧光免疫组化法等方法对诱导后的细胞进行签定;同时,记录各诱导组获得临床可用神经干细胞所需的时间及细胞数计数。结果证实各组均可使间充质干细胞(MSCs)定向分化为神经干细胞,所获得的细胞数亦可满足临床需要,但在所需的天数及具体获得细胞数量上各组间有一定差异,其中以患者BMSCs用自愿者脑脊液诱导组所获得神经干细胞计数最高,且其所需时间最短。
Looking for a stem cell acquisition pattern that suits the needs of patients and further promoting the application of neural stem cells to the clinic. In this experiment, allogeneic umbilical cord blood-derived mesenchymal stem cells (UMSCs) and bone marrow-derived mesenchymal stem cells (BMSCs) were induced to differentiate into neural stem cells in vitro from cerebrospinal fluid (CSF) of patients and their morphology, flow cytometry, immunohistochemistry , Fluorescence immunohistochemistry and other methods for the induction of cells after signing; the same time, each induction group to obtain clinically available neural stem cells required for the time and cell count. The results confirmed that each group can make mesenchymal stem cells (MSCs) differentiate into neural stem cells, the number of cells obtained can meet the clinical needs, but in the required number of days and the number of cells obtained specifically there are some differences between the groups, Among them, the patients with BMSCs induced by CSF of cerebrospinal fluid in volunteers had the highest counts of neural stem cells and the shortest time required.