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目的：观察果糖－１，６－二磷酸（ＦＤＰ）对急性冬眠心肌心功能和能量代谢的影响。方法：建立急性冬眠心肌模型，四道生理记录仪记录左心室内压力曲线、压力上升速率曲线；高效液相色谱法检测三磷酸腺苷及磷酸肌酸含量，蒽酮试剂检测心肌糖原含量，酶促偶联法测定灌流液中乳酸。结果：①冬眠９０分钟时，ＦＤＰ治疗组（ｎ＝７）心肌磷酸肌酸含量为３７．９１±２．９７μｍｏｌ／ｇ干重，明显高于冬眠组（ｎ＝７）的１５．７６±０．７６μｍｏｌ／ｇ干重（Ｐ＜０．０５）。②ＦＤＰ减少急性冬眠心肌舒张峰压上升幅度，在缺血３０分钟和６０分钟，ＦＤＰ治疗—再灌注组（ｎ＝６）明显低于冬眠—再灌注组（ｎ＝７，Ｐ＜０．０５）。冬眠期间，ＦＤＰ治疗—再灌注组左心室收缩峰压和左心室内压力最大上升速率均明显降低（Ｐ＜０．０５）。结论：ＦＤＰ可增加急性冬眠心肌的磷酸肌酸含量，改善心肌舒张功能，对冬眠心肌起一定的保护作用。
Objective: To observe the effects of fructose-1,6-diphosphate (FDP) on cardiac function and energy metabolism in acute hibernating myocardium. Methods: The model of acute hibernating myocardium was established. The left ventricular pressure curve and the rate of pressure rise were recorded by four physiological loggers. The contents of adenosine triphosphate and creatine phosphate were measured by high performance liquid chromatography. The antithrombin was detected by anthrone reagent. Determination of lactic acid in perfusate by the combined method. Results: ① At 90 minutes of hibernation, the creatine phosphorylation of FDP group (n = 7) was 37.91 ± 2.97μmol / g dry weight, significantly higher than that of hibernation group (n = 7) 15.76 ± 0 .76 μmol / g dry weight (P <0.05). (2) FDP decreased the increase of peak diastolic pressure in acute hibernating myocardium, and was significantly lower in FDP-reperfusion group (n = 6) than in hibernating-reperfusion group at 30 minutes and 60 minutes . Hibernation, FDP treatment - reperfusion group, left ventricular systolic pressure and left ventricular pressure peak rise rate were significantly lower (P <0.05). Conclusion: FDP can increase the content of creatine phosphokate in acute hibernating myocardium and improve myocardial diastolic function, which may play a protective role in hibernating myocardium.