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本研究旨在观察视网膜神经节细胞在高浓度葡萄糖下的视觉反应特性.实验中,视杯平铺于记录腔,Ames缓冲液灌流,单细胞外记录小鼠(Mus musculus)视网膜神经节细胞.实验结果表明,高糖条件下,ON神经节细胞的平均感受野大小(34.1±2.9,n=14)明显小于OFF神经节细胞的(49.3±0.3,n=12)(P<0.0001).高渗条件下,可以观察到类似的模式,即ON神经节细胞的平均感受野大小小于OFF的(P<0.0001).ON神经节细胞的平均亮度阈值在高糖(P<0.0001)或者高渗(P<0.0002)条件下明显升高.OFF神经节细胞的平均亮度阈值在相同条件下(高糖:P<0.01;高渗:P<0.0002)也有升高.在高渗条件下,ON神经节细胞的平均对比增益明显低于OFF神经节细胞的(P<0.015).而在高糖条件下,ON神经节细胞的平均增益明显高于OFF神经节细胞的(P<0.0001).这些结果表明,高糖可缩小神经节细胞的感受野,降低亮度敏感度,减弱对比增益.高糖对ON和OFF神经节细胞的影响可能是通过不同的机制进行的.
The purpose of this study was to observe the visual response of retinal ganglion cells under high concentration of glucose.In the experiment, cups were tiled in the recording chamber, Ames buffer was perfused, and retinal ganglion cells were recorded in a single extracellular. The experimental results showed that the mean sensory field size of ON ganglion cells (34.1 ± 2.9, n = 14) was significantly lower than that of the OFF ganglion cells (49.3 ± 0.3, n = 12) (P <0.0001) A similar pattern was observed under osmotic conditions in that the mean sensory field size of ON ganglion cells was smaller than OFF (P <0.0001). The mean brightness threshold of ON ganglion cells was significantly higher in hyperglycemic (P <0.0001) or hypertonic P <0.0002), the mean brightness threshold of OGF ganglion cells also increased under the same conditions (high glucose: P <0.01, hypertonicity: P <0.0002) The average contrast gain of cells was significantly lower than that of the OFF ganglion cells (P <0.015), while the average gain of ON ganglion cells was significantly higher than that of the OFF ganglion cells under high glucose conditions (P <0.0001). These results indicate , High glucose can reduce the sensory field of ganglion cells, reduce the brightness sensitivity, weaken the contrast gain.High sugar on ON and OFF God Impact of ganglion cells may have been made by different mechanisms.