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本文利用生物化学的手段,对大鼠进行了急性和亚急性毒性实验,研究溴氰菊酯对动物中枢神经系统离子调节作用的影响。急性实验结果表明:<1>溴氰菊酯能显著抑制脑微粒体上的Ca~(2+)+Mg~(2+)-ATP酶和Na~++K~+-ATP酶活性,但并不降低ecto-Ca~(2+)-ATP酶(细胞表面的Ca~(2+)-ATP酶)的活性;<2>溴氰菊酯对大鼠小脑组织中的环腺苷酸含量无明显影响,但却能显著升高与其作用相反的环鸟苷酸含量。体外实验证明,溴氰菊酯能够减少线粒体对Ca~(2+)的主动摄取。在对大鼠进行的亚急性实验中,发现溴氰菊酯中毒组与对照组大鼠的Ca~(2+)+Mg~(2+)-ATP酶、Na~++K~+-ATP酶和ecto-Ca~(2+)-ATP酶的活性均无显著性差异。根据以上结果推测,在急性中毒的条件下,溴氰萄酯能引起大鼠脑神经细胞内Ca~(2+)和Na~+的浓度增高,致使神经兴奋性发生改变。
In this paper, acute and subacute toxicity experiments were carried out in rats using biochemical methods to study the effects of deltamethrin on the ion regulation of the central nervous system in animals. Acute experimental results showed that: <1> Deltamethrin could significantly inhibit the activity of Ca ~ (2 +) + Mg ~ (2 +) - ATPase and Na ~ ++ K ~ + -ATPase in brain microsomes Did not decrease the activity of ecto-Ca ~ (2 +) - ATPase (Ca ~ (2 +) - ATPase on the cell surface); ②The activity of deltamethrin in rat cerebellum No significant effect, but significantly increased its role in the opposite of cyclic guanosine acid content. In vitro experiments show that deltamethrin can reduce mitochondrial Ca2 + uptake. In subacute rats, subgroups of Ca ~ (2 +) + Mg ~ (2 +) - ATPase, Na ~ ++ K ~ + -ATP There was no significant difference in the activity of enzyme and ecto-Ca ~ (2 +) - ATPase. According to the above results, it is speculated that under the condition of acute poisoning, cyanogen bromide can cause the increase of intracellular Ca2 + and Na ~ + concentration in rat brain cells, resulting in the change of nerve excitability.