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通过菌丝生长速率法和悬滴法测定ZJUF0986活性代谢产物对稻瘟病菌菌丝生长、孢子萌发和附着胞形成的影响。结果表明,ZJUF0986活性代谢产物对稻瘟病菌菌丝生长的有效中浓度EC50为18.55mg/L,与对照药剂三环唑的EC50(17.30mg/L)相比无显著性差异。活性代谢产物不仅影响孢子萌发,也显著降低附着胞的形成。浓度为10mg/L的活性代谢物可完全抑制孢子萌发及附着胞的形成;浓度为1.25mg/L时则明显延缓孢子萌发及附着胞的形成,处理48h后的孢子萌发率和附着胞形成率分别为62.17%和38.46%。以浓度为1.25mg/L活性代谢产物处理的稻瘟病菌孢子悬浮液接种离体大麦叶片,病原菌孢子在大麦叶片表面能部分萌发形成附着胞,但侵染栓形成延迟,致病性明显降低。
The mycelial growth rate and hanging drop method were used to determine the effects of ZJUF0986 active metabolites on the mycelial growth, spore germination and adherent formation of Magnaporthe grisea. The results showed that the effective medium concentration EC50 of ZJUF0986 active metabolites on the mycelial growth of Magnaporthe grisea was 18.55 mg / L, which was not significantly different from that of the control drug tricyclazole EC50 (17.30 mg / L). Active metabolites not only affect spore germination, but also significantly reduce the formation of attachment cells. The concentration of 10mg / L of the active metabolite can completely inhibit the spore germination and the formation of attached cells; concentration of 1.25mg / L significantly delayed spore germination and attachment formation, spore germination rate and attachment formation rate after 48h treatment Respectively 62.17% and 38.46%. Inoculated spore suspension of Magnaporthe grisea with Magnaporthe grisea with a concentration of 1.25 mg / L of active metabolite inoculated on the surface of barley leaves, the pathogen spores germinated to form attachment cells on the surface of the barley leaves. However, the infection plug was delayed and the pathogenicity was significantly reduced.