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目的探讨重组Endostatin基因(EScDNA)对大鼠胶质瘤的治疗效果及肿瘤微血管密度的变化。方法对质粒包埋的Endostatin基因(pSecTagA-EScDNA)测序鉴定后,转染人脐静脉内皮细胞(ECV-304),验证Endostatin基因对血管内皮细胞增殖的抑制作用;以C6胶质瘤细胞植于SD大鼠腋部皮下制作胶质瘤模型,将pSecTagA-EScDNA直接瘤内注射。分别观察记录肿瘤体积,绘制肿瘤生长曲线;对肿瘤标本进行微血管标记、计数,并比较治疗前后肿瘤微血管数量的变化。结果重组Endostatin基因治疗后的肿瘤生长明显受抑制,使肿瘤退缩至休眠甚至完全消失;肿瘤微血管密度明显低于未治疗者。结论重组Endostatin基因通过抑制肿瘤血管新生对大鼠胶质瘤有良好的治疗效果。
Objective To investigate the therapeutic effect of recombinant Endostatin gene (EScDNA) on glioma and the change of tumor microvessel density. Methods Endostatin gene (pSecTagA-EScDNA) was identified by sequencing and transfected into human umbilical vein endothelial cells (ECV-304) to verify the inhibitory effect of Endostatin gene on the proliferation of vascular endothelial cells. C6 glioma cells Glioma models were made subcutaneously in the axilla of SD rats, and the pSecTagA-ES cDNA was directly injected intratumorally. The tumor volume was observed and recorded, and the tumor growth curve was drawn. The tumor samples were labeled with microvessels and counted, and the changes of tumor microvessels before and after treatment were compared. Results The growth of tumor after recombinant Endostatin gene was obviously inhibited, which made the tumor retreat to or even completely disappear. The microvessel density of the tumor was obviously lower than that of the untreated group. Conclusion The recombinant Endostatin gene has a good therapeutic effect on rat glioma by inhibiting tumor angiogenesis.