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目的观察蛇毒出血毒素结构的变化对功能的影响。方法利用傅利叶变换红外光谱仪对尖吻蝮蛇毒出血毒素（ＤａＨＴ－３）在溶液中酰胺Ｉ带吸收光谱的研究，探测了此出血毒素在溶液中的自然构象和加入ＥＤＴＡ螯合剂除去金属离子后构象的变化。结果此出血毒素在水溶液中的自然构象分别是：α－螺旋为３１．８％、β－折叠为５６．１％、转角为１２．１％；而在去除金属离子情况下α－螺旋和β－折叠减少，转角和无规卷曲增加，即加入螯合剂后其α－螺旋、β－折叠、转角和无规卷曲分别变为１１％、２６．４％、４６．２％和１６．５％。由于结构的变化，它的出血活性和蛋白水解酶活性均被丧失。结论金属离子，特别是锌离子在维系蛇毒出血蛋白酶分子中的二级结构中起着很重要的作用。
Objective To observe the effect of changes of venom toxin structure on the function of venom. Methods Fourier transform infrared spectroscopy (FTIR) was used to study the absorption spectra of amide I band in solution of Daitake Agkistrodon halysii venom (DaHT-3) in solution. The natural conformation of the hemorrhagic toxin in solution and the conformation The change. Results The natural conformation of this hemorrhagic toxin in aqueous solution was 31.8% for α-helix, 56.1% for β-sheet, and 12.1% for the angle of rotation. In the case of removing metal ions, α-helix and β Fold reduction, corner and random coil increase, ie the alpha-helix, beta sheet, corner and random coil become 11%, 26.4%, 46.2% and 16.5% after addition of chelator, . Due to structural changes, its hemorrhagic activity and proteolytic enzyme activity were lost. Conclusions Metal ions, especially zinc, play an important role in maintaining the secondary structure in the venom protease.