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目的:探讨恩度对于SGC-7901人胃癌细胞的杀伤效应及机制。方法:应用MTT法观察恩度作用对SGC-7901人胃癌细胞的抑制效应;采用ANNEXIN-V染色法研究恩度对于SGC-7901人胃癌细胞的凋亡诱导作用;采用免疫细胞化学染色法研究恩度作用后Bcl-2及Bax蛋白表达的变化。结果:不同浓度的恩度对SGC-7901细胞的增殖抑制率为21.5%～57.8%(P<0.01),抑制效应随着浓度增加和作用时间延长而增强;各实验浓度的恩度均能诱导细胞凋亡发生,0.1、1.0、5.0、10.0和15.0mg/L恩度作用48h后SGC7901细胞的总死亡率分别为1.4%、27.2%、33.4%、43.5%、54.4%和67.0%;恩度作用后导致Bcl-2蛋白表达水平下降,而对于Bax蛋白无显著影响。结论:恩度通过诱导SGC-7901细胞发挥其抑制效应,其可能机制为抑制抗凋亡蛋白Bcl-2的表达。
Objective: To investigate the effect and mechanism of Endue on SGC-7901 human gastric cancer cells. Methods: MTT assay was used to observe the inhibitory effect of Endur effect on SGC-7901 human gastric cancer cells. The apoptosis of SGC-7901 human gastric cancer cells was investigated by ANNEXIN-V staining. The immunocytochemical staining Changes in the expressions of Bcl-2 and Bax proteins after treatment with Results: The inhibitory rates of different concentrations of Endu on the proliferation of SGC-7901 cells were 21.5% -57.8% (P <0.01), and the inhibitory effects were enhanced with the increase of concentration and duration of action; The apoptosis rates of SGC7901 cells were 1.4%, 27.2%, 33.4%, 43.5%, 54.4% and 67.0% respectively after 48h treatment with 0.1, 1.0, 5.0, 10.0 and 15.0mg / After treatment, Bcl-2 protein expression decreased, but no significant effect on Bax protein. Conclusion: Endostar can induce SGC-7901 cells to exert its inhibitory effect by inhibiting the expression of anti-apoptotic protein Bcl-2.