研究了影响大麦花药离体培养反应的一些因素。结果表明 :供体穗子经 3℃预处理可以促进花药离体培养反应 ,迟钝基因型的最佳处理时间比敏感基因型的短 ,以 10～ 15d为宜。诱导培养基中添加 80 0mg/LCH能够较大幅度提高花药反应率和绿苗率 ,在含CH的培养基内添加 50 0mg/L脯氨酸或 10 0mg/L精胺 ,均能进一步提高迟钝基因型的反应花药率 ,特别是绿苗率。 Some factors affecting the response of barley anther culture in vitro were studied. The results showed that donor panicle pre-treatment at 3 ℃ could promote the anther culture in vitro, and the optimal treatment time of the delayed genotype was shorter than that of the susceptible genotype. The addition of 80 0mg / LCH to the induction medium could greatly increase the antitumor response rate and green shoot rate, and the addition of 50mg / L proline or 100mg / L spermine in the medium containing CH could further increase the retardation Genotype response anther rate, especially green shoot rate.