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从我国山东东亚马氏钳蝎（ＢｕｔｈｕｓｍａｒｔｅｎｓｉＫａｒｓｃｈ）尾腺中分离纯化ｍＲＮＡ，经逆转录构建了ＢｍＫ蝎毒ｃＤＮＡ文库。利用ＰＣＲ扩增，筛选到两个抗哺乳动物毒素的ｃＤＮＡ基因，并测定了序列。这两个ｃＤＮＡ阅读框均为２５２ｂｐ组成，可翻译８４肽的毒素前体，包括Ｎ端１９个氨基酸组成的信号肽，６４个氨基酸残基的成熟毒蛋白，以及Ｃ端一个额外的碱性氨基酸Ａｒｇ。其中由一ｃＤＮＡ所推导的氨基酸序列（ＢｍＫＭ１）与已知的天然毒素ＢｍＫ１蛋白序列完全相同；而另一推导的氨基酸序列（ＢｍＫＭ９）与已知的天然毒素ＢｍＫＭ４有很大的同源性，其分子间仅有两个残基的变异。ＢｍＫＭ１与非洲蝎ＬｑｈαＩＴ的ｃＤＮＡ同源性高达８７．２％。
The mRNA of BmK scorpion venom was constructed by reverse transcription from the cauda glands of Buthus martensi Karsch in Shandong, China. Using PCR amplification, two cDNA genes against mammalian toxins were screened and sequenced. Both cDNAs are 252 bp in length and can translate the toxin of the 84 peptide, including the signal peptide consisting of the N-terminal 19 amino acids, the mature toxin of 64 amino acid residues, and an additional basic amino acid at the C-terminal Arg. The amino acid sequence deduced from a cDNA (BmKM1) is exactly the same as the known BmK1 protein sequence of native toxin. The other deduced amino acid sequence (BmKM9) has a great homology with the known natural toxin BmKM4, Only two residues between the molecular variation. The homology between BmKM1 and LqhαIT in African scorpion was as high as 87.2%.