目的研究藁本内酯脂质体最佳制备工艺及处方。方法采用乙醇注入法制备藁本内酯脂质体,以包封率为评价指标,采用正交设计优化处方和制备工艺,高效液相色谱法测定其包封率。结果最佳工艺处方为:藁本内酯与卵磷脂的比例为1∶10,卵磷脂与胆固醇的质量比为2∶1,PBS缓冲溶液pH为7.5,制备温度为35℃。结论经优化得到的藁本内酯脂质体处方合理,工艺可行。 Objective To study the optimal preparation and prescription of ligustilide liposomes. Methods The ligustilide liposomes were prepared by ethanol injection. The encapsulation efficiency was used as the evaluation index. Orthogonal design was used to optimize the formulation and preparation process. The entrapment efficiency was determined by high performance liquid chromatography. Results The best prescription was that the ratio of ligustilide to lecithin was 1:10, the mass ratio of lecithin to cholesterol was 2:1, the pH value of PBS buffer solution was 7.5 and the preparation temperature was 35 ℃. Conclusion The optimized ligustilide liposomes prescription is reasonable and the technology is feasible.