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用SPA-ELISA一步法检测了囊虫病患者血清特异性抗体,并与快速SPA-ELISA进行对比。两法同时检测了59例囊虫病患者,36例非囊虫病病人和30例正常人血清。结果表明:SPA-ELISA一步法和快速SPA-ELISA的阳性率分别为94.91%和98.31%,几何平均滴度(GMP)分别为1∶425.89和1∶565.72,均无显著性差异。对1例肝吸虫病人和1例包虫病人血清均起交叉反应。说明SPA-ELISA一步法具有与快速SPA-ELISA相同敏感性和特异性,并且快速、方便、花费少,可作为囊虫病人的诊断及流行病学调查的方法
Serum-specific antibodies against cysticercosis were detected by SPA-ELISA in one step and compared with rapid SPA-ELISA. In the two methods, 59 patients with cysticercosis, 36 patients with non-cysticercosis and 30 normal persons were detected simultaneously. The results showed that the positive rates of SPA-ELISA and SPA-ELISA were 94.91% and 98.31% respectively, and the geometric mean titers (GMP) were 1: 425.89 and 1: 565.72 respectively No significant difference. Serum samples from 1 patient with liver flu and 1 patient with hydatid disease all cross-reacted. It shows that the SPA-ELISA one-step method has the same sensitivity and specificity as rapid SPA-ELISA, and is fast, convenient and low-cost and can be used as a diagnostic and epidemiological investigation method for cysticercosis patients