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目的建立从草苁蓉和肉苁蓉中提取总苷的含量测定方法,并对草苁蓉和肉苁蓉中的总苷含量进行测定。方法选择红景天苷作为对照品,使用香草醛-高氯酸溶液进行显色反应,用分光光度法在523nm波长下对草苁蓉和肉苁蓉中总苷的含量进行测定。结果红景天苷在0.05133~0.2567mg·ml~(-1)线性关系良好,回归方程Y=1.611X+0.0573(r=0.9992),草苁蓉的平均回收率为99.68%,RSD 1.68%,肉苁蓉的平均回收率为101.18%,RSD为0.94%。结论该方法精密度较高、简便、稳定性好,可用于草苁蓉和肉苁蓉中总苷含量的测定。
OBJECTIVE To establish a method for the determination of total glycosides extracted from Cistanche desertica and Cistanche tubulosa and to determine the content of total glycosides in Cistanche desertica and Cistanche desertica. Methods Salidroside was selected as the reference substance, and the color reaction was carried out using vanillin-perchloric acid solution. The content of total glycosides in Cistanche desertica and Cistanche tubulosa were determined by spectrophotometry at 523nm wavelength. Results The salidroside had a good linearity in the range of 0.05133 ~ 0.2567mg · ml -1. The regression equation was Y = 1.611X + 0.0573 (r = 0.9992). The average recovery rate was 99.68% and the RSD was 1.68% The average recovery rate of Cistanche was 101.18% with a RSD of 0.94%. Conclusion The method has high precision, simpleness and good stability, and can be used for the determination of total glycosides in the grass of Cistanche desertica and Cistanche tubulosa.