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目的:研究miR-106b在卵巢浆液性癌中的表达及其对卵巢癌细胞增殖、侵袭的影响,并对其可能机制进行初步分析。方法:运用原位杂交和RT-PCR技术检测输卵管上皮和卵巢浆液性癌组织中miR-106b表达情况;转染miR-106b的mimics作用于SKOV3细胞,应用生长曲线、克隆形成、侵袭实验检测miR-106b对细胞增殖和侵袭的影响。结果:原位杂交实验显示,miR-106b在卵巢浆液性癌组织中的表达明显高于输卵管上皮。Real time-PCR结果证实,miR-106b在多数卵巢浆液性癌患者(n=30)组织中的表达明显高于输卵管上皮(n=8);生长曲线和克隆形成试验显示,过表达miR-106b明显促进卵巢癌细胞的增殖和转化;侵袭实验显示,miR-106b明显促进卵巢癌细胞的侵袭力。结论:miR-106b在卵巢浆液性癌组织中高表达,miR-106b促进卵巢癌细胞的增殖、克隆形成和侵袭,miR-106b在卵巢浆液性癌发生发展中可能发挥重要作用。
OBJECTIVE: To study the expression of miR-106b in ovarian serous carcinoma and its effect on the proliferation and invasion of ovarian cancer cells, and to analyze its possible mechanism. Methods: The expression of miR-106b in ovarian epithelial and ovarian serous carcinoma tissues was detected by in situ hybridization and RT-PCR. The mimics transfected with miR-106b were applied to SKOV3 cells. The growth curve, clonogenicity and invasion assay were used to detect miR -106b on cell proliferation and invasion. Results: In situ hybridization experiments showed that the expression of miR-106b in ovarian serous carcinoma tissue was significantly higher than the tubal epithelium. The results of Real time-PCR confirmed that the expression of miR-106b was significantly higher in the majority of ovarian serous carcinoma tissues (n = 30) than in the oviduct epithelium (n = 8). The growth curve and clonogenic assay showed that overexpression of miR- Significantly promote ovarian cancer cell proliferation and transformation; invasion experiments showed that miR-106b significantly promote the invasiveness of ovarian cancer cells. Conclusion: miR-106b is overexpressed in serous ovarian cancer tissues. MiR-106b promotes the proliferation, colony formation and invasion of ovarian cancer cells. MiR-106b may play an important role in the development of ovarian serous carcinoma.