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水稻Rim2/Hipa是最近鉴定的一个受逆境诱导的转座因子超级家族.研究表明,Rim2的核心序列在不同来源的水稻材料中存在显著的差异,暗示Rim2家族的长期进化历程.基于Rim2因子间的差异性以及该因子的静止状态,开发出一种利用Rim2因子展示的新的分子指纹技术,可以灵敏地区分不同水稻资源以及它们的遗传关系.仅用5对引物就可以清楚地将53个栽培稻和普通野生稻材料鉴定出来,并可将它们分为不同的系统进化组.研究表明不仅在水稻资源而且在野生稻种质间均存在明显的多样性.野生稻可以被单独分组,或者分散在粳稻中间.这种新的指纹技术还可以将水稻的杂交子代和它们的亲本区分出来,并可用于种子纯度的鉴定,在水稻基因组进化研究、水稻育种和种子生产中有很好的应用前景.
Rice Rim2 / Hipa is a newly identified transcontinental transposon superfamily. Studies have shown that there is a significant difference in the core sequence of Rim2 among different rice sources, suggesting a long-term evolutionary history of the Rim2 family. And the quiescence of this factor, a new molecular fingerprinting technique developed using the Rim2 factor was developed to differentiate sensitive rice resources and their genetic relationships, using only 5 pairs of primers to clearly distinguish 53 Cultivated rice and common wild rice, and can be divided into different phylogenetic groups.The research shows that there are obvious diversity not only in rice resources but also in wild rice germplasm.The wild rice can be grouped separately or Scattered in the middle of japonica rice.This new fingerprinting technology can also distinguish the hybrid progenies of rice and their parents and can be used for the identification of the purity of the seeds and has good results in rice genome evolution research, rice breeding and seed production Application prospects.